Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain X-33 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
T223V | the mutation removes the potential glycosylation site and shows reduced enzyme activity | Homo sapiens |
T223V/DELTAE286-E289 | the mutation shows reduced enzyme activity | Homo sapiens |
T223V/DELTAE286-N293 | the mutation shows reduced enzyme activity | Homo sapiens |
T223V/M274L/D275N/G277A/V278M | the mutation shows reduced enzyme activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
E-64 | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | wild type enzyme | Homo sapiens | |
0.0014 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | mutant enzyme T223V/DELTAE286-E289 | Homo sapiens | |
0.0015 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | mutant enzyme T223V/M274L/D275N/G277A/V278M | Homo sapiens | |
0.0017 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | mutant enzyme T223V/DELTAE286-N293 | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
recombinant enzyme, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P07711 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Phenyl-Sepharose column chromatography, and SP-Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-casein + H2O | - |
Homo sapiens | ? | - |
? | |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cathepsin-L | - |
Homo sapiens |
cathepsin-L T2V | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | mutant enzyme T223V/M274L/D275N/G277A/V278M | Homo sapiens | |
18 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | mutant enzyme T223V/DELTAE286-E289 | Homo sapiens | |
30 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | mutant enzyme T223V/DELTAE286-N293 | Homo sapiens | |
46 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | wild type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | - |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | - |
Homo sapiens |